[Answer] What does the salt in the extraction buffer do?

Answer: It will help break up protein chains that bind around the nucleic acid.
What does the salt in the extraction buffer do?

A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein or for DNA extraction). Most lysis buffers contain buffering salts (e.g. Tris-HCl) and ionic salts (e.g. NaCl) to regulate the pH and osmolarity of the lysate.

Sometimes detergents (such as Triton X-100 or SDS) are added to break up membrane structures. For lysis buffers targeted at protein extraction protease inhibitors are often included and in difficult cases may be almost required. Lysis buffers can be used on both animal and plant tissue cells.

Sometimes detergents (such as Triton X-100 or SDS) are added to break up membrane structures. For lysis buffers targeted at protein extraction protease inhibitors are often included and in difficult cases may be almost required. Lysis buffers can be used on both animal and plant tissue cells.

Wed May 07 2008 14:30:00 GMT-0400 (Eastern Daylight Time) · This buffer is intended to maintain binding conditions while removing the binding salts and other remaining contaminants. Generally it takes several washes often with increasing percentages of ethanol/isopropanol until the nucleic acid on the silica membrane is free of contaminants.

Lysis buffer – Wikipedia

Salting out – Wikipedia

Spin column-based nucleic acid purification – Wikipedia

Salting out – Wikipedia

Phosphate-buffered saline is a buffer solution commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate sodium chloride and in some formulations potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH. The osmolarity and ion concentrations of the solutions match those …

Tue Sep 07 2004 14:30:00 GMT-0400 (Eastern Daylight Time) · Salting out is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength. Salting out is typically used to precipitate large biomolecules such as proteins or DNA. Because the salt concentration needed for a given protein to precipitate out of the solution diffe…

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