[Answer] In the experiment shown why did the researchers have to attach a general-purpose promoter to the cDNAs (Step 3)?

In the experiment shown why did the researchers have to attach a general-purpose promoter to the cDNAs (Step 3)?

In molecular biology the TATA box (also called the Goldberg-Hogness box) is a sequence of DNA found in the core promoter region of genes in archaea and eukaryotes. The bacterial homolog of the TATA box is called the Pribnow box which has a shorter consensus sequence.. The TATA box is considered a non-coding DNA sequence (also known as a cis-regulatory element).

A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface.Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles (10 −12 moles) of a specific DNA sequence known as probes (or reporters or …

DNA methylation is a biological process by which methyl groups are added to the DNA molecule. Methylation can change the activity of a DNA segment without changing the sequence. When located in a gene promoter DNA methylation typically acts to repress gene transcription.In mammals DNA methylation is essential for normal development and is ass…