[Answer] how does substrate concentration affect enzyme activity?

Answer: increase initially and then level off because all enzymes are constantly occupied (rate then depends on how quickly catalysis occurs)
how does substrate concentration affect enzyme activity?

What are Enzymes? (with pictures) – wisegeek.com

What Is the Connection between Enzymes and Substrates?

Several factors effect the assay outcome and a recent review summarizes the various parameters that needs to be monitored to keep an assay up and running. • Salt Concentration: Most enzymes cannot tolerate extremely high salt concentrations. The ions interfere with the weak ionic bonds of proteins. Typical enzymes are active in salt concentrations of 1-500 mM. As usual there are exceptions such as the halophilic algae and bacteria.

Enzyme kinetics is the investigation of how enzymes bind substrates and turn them into products. The rate data used in kinetic analyses are commonly obtained from enzyme assays. In 1913 Leonor Michaelis and Maud Leonora Menten proposed a quantitative theory of enzyme kinetics which is referred to as Michaelis–Menten kinetics . The major contribution of Michaelis and Menten was to think of enzyme reactions in two stages. In the first the substrate binds reversibly to the enzyme forming the enzyme-substrate complex. This is sometimes called the Michaelis–Menten complex in their honor. Th…

Enzyme kinetics is the investigation of how enzymes bind substrates and turn them into products. The rate data used in kinetic analyses are commonly obtained from enzyme assays. In 1913 Leonor Michaelis and Maud Leonora Menten proposed a quantitative theory of enzyme kinetics which is referred to as Michaelis–Menten kinetics. The major contribution of Michaelis and Menten was to think of enzyme reactions in two stages. In the first the substrate binds reversibly to the enzyme forming the enzyme-substrate complex. This is sometimes called the Michaelis–Menten complex in their honor. The enzyme then catalyzes the chemical step in the reaction and releases the product. This work was further developed by G. E. Briggs and J. B. S. Haldane who derived kinetic equations that are still widely used today. Enzyme rates depend on solutio…

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